cytof batch (CellCarta)

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CellCarta cytof batch
Cytof Batch, supplied by CellCarta, used in various techniques. Bioz Stars score: 99/100, based on 184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cytof+batch/CellEngine/pm39120971-731-59-71
Average 99 stars, based on 184 article reviews

cytof batch - by Bioz Stars, 2026-10

99/100 stars

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Article Title: Multimodal analysis of dysregulated heme metabolism, hypoxic signaling, and stress erythropoiesis in Down syndrome.
Article Snippet: .. For pre-processing, bead-based normalization via polystyrene beads embeddedwith lanthanides, bothwithin and between batches, followed by bead removal was carried out using theMatlab-based Normalizer tool.77 Batched FCS files were demultiplexed using the Matlab-based Single Cell Debarcoder tool.78 Reference-based normalization of individual samples across batches against the common reference sample was then carried out using the R script BatchAdjust() from the CyTOF Batch Adjust package.79 For the analyses described in this manuscript, CellEngine (CellCarta) was used to gate and export per-sample FCS files at four levels: Firstly, CD3+CD19+ doublets were excluded, and remaining cells exported as ‘Live’ cells; Live cells were then gated for hematopoietic lineage (CD45-positive) non-granulocytic (CD66-low) cells and exported as CD45+CD66low. .. For pre-processing, bead-based normalization via polystyrene beads embeddedwith lanthanides, bothwithin and between batches, followed by bead removal was carried out using theMatlab-based Normalizer tool.77 Batched FCS files were demultiplexed using the Matlab-based Single Cell Debarcoder tool.78 Reference-based normalization of individual samples across batches against the common reference sample was then carried out using the R script BatchAdjust() from the CyTOF Batch Adjust package.79 For the analyses described in this manuscript, CellEngine (CellCarta) was used to gate and export per-sample FCS files at four levels: Firstly, CD3+CD19+ doublets were excluded, and remaining cells exported as ‘Live’ cells; Live cells were then gated for hematopoietic lineage (CD45-positive) non-granulocytic (CD66-low) cells and exported as CD45+CD66low.

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